polyclonal rabbit anti rat pcna  (Danaher Inc)

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats

doi: 10.3892/etm.2015.2592

Figure Lengend Snippet: Number of white blood cells in the peripheral blood (x10 9 /l) and the ratio of CD34 + cells among the mononuclear cells (%).

Article Snippet: Rabbit anti-rat CD34 + polyclonal antibody (dilution, 1:200; cat. no. bs-0754R; Beijing Bioss Biosynthesis Biotechnology Co., Ltd.) was used as the primary antibody for overnight incubation at 4°C.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats

doi: 10.3892/etm.2015.2592

Figure Lengend Snippet: Number of cluster of differentiation 34+ cells in four groups of rat renal tissues (arrows indicate immunohistochemical staining; magnification, x200). (A–C) The (A) normal control, (B) treatment control and (C) model groups at day 5; (D–F) the model group at (D) day 10, (E) day 17 and (F) day 24; (G–J) the treatment group at (G) day 5, (H) day 10, (I) day 17 and (J) day 24.

Article Snippet: Rabbit anti-rat CD34 + polyclonal antibody (dilution, 1:200; cat. no. bs-0754R; Beijing Bioss Biosynthesis Biotechnology Co., Ltd.) was used as the primary antibody for overnight incubation at 4°C.

Techniques: Immunohistochemical staining, Staining

Journal: Cardiovascular Diagnosis and Therapy

Article Title: Inhibition of ERK or Akt ameliorates intimal hyperplasia via up-regulation of Cx37 and down-regulation of Cx43 in balloon injury rat model

doi: 10.21037/cdt-20-345

Figure Lengend Snippet: Inhibition of ERK and Akt reduced neointima formation and cell proliferation in the balloon-injured rat model. (A) Representative H&E staining photomicrographs of carotid arteries for NI rats and untreated, MK2206-treated, and U0126-treated BI rats on day 14 surgery. Magnification 200×. (B) Quantification of the intima-to-media ratio. (C) Representative PCNA immunostaining 14 days post carotid ligation in NI, BI, MK2206-treated BI and U0126-treated BI rats. Magnification 200×. (D) Quantitative analysis of the luminal area. (E) Quantitative assessment of PCNA positive area over the total carotid area. *, P<0.05; **, P<0.01, versus BI rats. #, P<0.05; ##, P<0.01, versus NI rats. Bar graph indicates the mean ± SEM. BI, balloon injury; NI, not injured; PCNA, proliferating cell nuclear antigen.

Article Snippet: Microwave-heating was applied for antigen retrieval, and nonspecific blocking was performed by 4% normal goat serum plus 1% bovine serum albumin (BSA) in phosphate-buffered saline for 30 min. Then, an anti-rat PCNA rabbit polyclonal antibody (1:100; Santa Cruz Biotechnology Inc., Santa Cruz, CA) was used to incubate the sections.

Techniques: Inhibition, Staining, Immunostaining, Ligation

Journal: BioMedicine

Article Title: The potential therapeutic effect for melatonin and mesenchymal stem cells on hepatocellular carcinoma

doi: 10.1051/bmdcn/2019090424

Figure Lengend Snippet: Photomicrographs of liver sections immuostained with PCNA antibody in normal (A), HCC (B), Mel (C), MSCs (D), and Mel + MSCS (E) groups. Scale bar = 50 μm.

Article Snippet: Immunostaining was performed as previously described [ , ] using polyclonal rabbit anti-rat PCNA antibodies (1:500 dilution, Thermo-Scientific, USA) and goat anti-rabbit secondary antibody (1:1000 dilution, Dako, USA).

Techniques:

Journal: Molecular Medicine Reports

Article Title: Autologous adipose tissue-derived mesenchymal stem cells are involved in rat liver regeneration following repeat partial hepatectomy

doi: 10.3892/mmr.2016.4768

Figure Lengend Snippet: Effect of ADSC transplantation on the PCNA-labeling index at 24, 72, and 168 h postoperatively. Data are expressed as the mean ± standard deviation of the results from five animals. * P<0.01, vs. PH group; ** P<0.01, vs. R-PH group. ADSC, adipose tissue-derived mesenchymal stem cell; PCNA, proliferating cell nuclear antigen; PH, partial hepatectomy; R-PH, repeat PH.

Article Snippet: Briefly, following fixation with formalin and paraffin embedding, the liver tissue sections were incubated with rabbit anti-rat polyclonal antibody against PCNA (cat. no. GTX100539; 1:500 dilution; Genetex Inc. Irvine, CA, USA) at 4°C overnight, and subsequently with a 3′,3-diaminobenzidine kit (Beyotime Institute of Biotechnology, Haimen, China).

Techniques: Transplantation Assay, Labeling, Standard Deviation, Derivative Assay